Core Facilities of School of Life Sciences, aiming to
manage public equipments of our school, was officially put to operation in
April, 2010 upon a decision of establishment in September, 2009.
Equipment and Staff
At present, there are 7 equipment management specialists
and 2 postdoctoral fellow at Core Facilities. Its large-scale equipments can be
categorized into the following platforms as below:
• DNA sequencing platform: Sanger sequencing
on ABI3730xl DNA Analyzer and some associate facilities for wide variety
of sequencing services.
• Electron microscopy platform: Including FEI
Tecnai G2 20 Transmission Electron Microscope (TEM) and seven specimen
preparation apparatuses such as ultramicrotomes (Leica UC7/FC7), glass knife
maker, sputter coater, freeze substitution system, bechtop plasma cleaner
and automated routine tissue processor.
• Bioimaging platform: 7 sets of bioimaging
facility, containing: Zeiss LSM-710NLO &DuoScan two-photon laser scanning
confocal microscopy; API DeltaVision live cell system, PE UltraVIEWVoX
spinning-disk confocal microscopy; MD ImageXpressMicroXL high content live cell
imaging microscopy; IONSCOPE ICnanoS2 scanning ion conductance microscopy;
Nikon N-SIM super resolution microscopy; Nikon N-STORM super resolution
microscopy, and Bitplane Imaris multidimensional image processing and analyzing
• Flow cytometry platform: Including
MoFloXDP high-speed cell sorting system (Beckman Coulter) and BD FACSCalibur
flow cytometry. Other two flow cytometers on the way.
• Protein platform: Including Real-time
quantitative PCR detecting system; Cell disruptor system; Surface plasmon
resonance protein analyzer; Protein purification chromatography system;
Circular Dichroismchiroptical spectrometer and so on.
• Centrifuge platform: Including High Speed
Refrigerated Centrifuges; Preparative ultracentrifuges and Refrigeration Dryer;
• Isotope Laboratory: To serve and help all
users work with radioactive materials or machines in a safe and secure manner.
Core Facilities is undertaking the operation management
of bioimaging platform and protein platform from National Center for
Protein Science (PHOEIX). Five large-scale imaging devices (optical tweezers,
etc) will be purchased to the two platforms along with ELISA analysis and
detection system, incubated stackable shakers，high capacity
incubators, WAVE bioreactors, etc.